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Applied Biological Chemistry

Table 4 Linearity and LOQ for formononetin (1) and biochanin A (2)

From: Validation of an optimized HPLC–UV method for the quantification of formononetin and biochanin A in Trifolium pratense extract

Compound

tR

Range (mg/mL)

Calibration equationa

r2b

LOQ (mg/mL)

1

31.8

0.125–0.00781

Y = 5002.2X + 58.6

0.999

0.0160

2

36.3

0.125–0.00781

Y = 7265.0X + 109

0.999

0.0190

  1. aY = peak area, X = concentration of standard compounds (mg/mL)
  2. br2 = correlation coefficient for five calibration data points (n = 5)
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