Table 1 Purification table for the CDH extracted from solid-state cultures of Coprinellus aureogranulatus (CauCDH)
Purification steps | Total activity, U | Total protein, mg | Specific activity, U mg−1 | Yield, % | Purification, fold |
|---|---|---|---|---|---|
Crude extract | 1817 | 2636 | 0.7 | 100 | 1.0 |
Ultrafiltration | 1687 | 2239 | 0.8 | 92.8 | 1.1 |
DEAE Sepharose | 889 | 232 | 3.8 | 48.9 | 5.6 |
Superdex™ 75 | 549 | 26 | 21.3 | 30.2 | 30.9 |
HiTrap™ Q FF | 412 | 14 | 28.9 | 22.7 | 41.9 |