Table 2 Substrate specificity of CauCDH
Substrates* | Specific activity (U mg−1) | Km (µM) | kcat (s−1) | kcat/Km (M−1 s−1) |
|---|---|---|---|---|
Cellobiose | 19.2 | 117.1 | 30.2 | 25.8 × 104 |
Cellotriose | 16.9 | 205.5 | 26.6 | 12.9 × 104 |
Cellotetraose | 17.1 | 232.0 | 26.8 | 11.6 × 104 |
Lactose | 39.3 | 731.1 | 61.7 | 84.5 × 103 |
Glucose | 5.7 | 49,200 | 8.9 | 180.3 |
Maltose | 18.7 | 1,354,000 | 29.4 | 21.7 |