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Applied Biological Chemistry

Table 3 Kinetic constants of CauCDH towards various electron acceptors

From: Cellobiose dehydrogenase from the agaricomycete Coprinellus aureogranulatus and its application for the synergistic conversion of rice straw

Electron acceptors*

Stoichiometry** (# of e−)

Km (μM)

kcat (s−1)

kcat/Km (M−1 s−1)

cyt c

2

1.3

30.1

23.2 × 106

DCIP

2

1.6

30.0

18.4 × 106

ABTS•+

1

15.6

47.4

30.4 × 105

Potassium ferricyanide

2

18.5

37.7

20.4 × 105

1,4-Benzoquinone

1

37.1

25.2

67.9 × 104

2,6-Dimethyl-1,4-benzoquinone

1

292.2

19.3

66.1 × 103

2,6-Dimethoxy-1,4-benzoquinone

1

325.5

11.4

35.0 × 103

  1. *Cellobiose (20 mM) was used as the electron donor
  2. **Moles of electron acceptor reduced per mole of cellobiose oxidized ABTS: 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
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