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Applied Biological Chemistry

Table 1 Linearity (r2), limit of detection (LOD), and limit of quantification (LOQ) of shikimic acid, p-coumaric acid, and sinapinic acid analysed using GC-TOFMS and GC  ×  GC-TOFMS

From: Improved annotation and quantification of metabolites in rice (Oryza sativa L.) seeds using two-dimensional gas chromatography–time-of-flight mass spectrometry

Metabolite

Calibration curve

Linearity

LOD (ng)

LOQ (ng)

GC-TOFMS

 Shikimic acid

y  =  0.0529x  −  0.0054

0.9963

9.26

30.88

 p-Coumaric acid

y  =  0.0080x  −  0.0083

0.9910

90.18

300.60

 Sinapinic acid

y  =  0.0035x  −  0.0025

0.9920

119.58

398.59

GC  ×  GC-TOFMS

 Shikimic acid

y  =  0.1486x  +  0.0004

0.9978

0.31

1.03

 p-Coumaric acid

y  =  0.0221x  −  0.0022

0.9987

55.87

186.25

 Sinapinic acid

y  =  0.0176x  −  0.0078

0.9966

47.22

157.41

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