Fig. 3

EA inhibited HSFs contraction in a dose-dependent manner. HSFs contraction in different experimental groups, as detected using a collagen lattice contraction assay after 48 h of treatment (n = 5). A Representative pictures, and B quantitative analysis of contraction rate (%) and inter-group comparison are shown. C Relative mRNA level of ACTA2 in different experimental groups, as detected using qPCR (n = 5). D Protein levels of α-SMA and GAPDH in different experimental groups, as detected using western blotting, data were collected from three independent experiments. Data are presented as mean ± SD; *P < 0.05 and **P < 0.01