Fig. 6
EA inhibited TGF-β1-induced up-regulation of HSFs proliferation, migration, and contraction, and collagen synthesis in HSFs. A HSFs proliferation detected using CCK-8 assay; HSFs migration detected using B wound healing assay (scale bar = 200 μm) and C transwell assay (scale bar = 100 μm); D HSFs contraction detected using collagen lattice contraction assay; E relative mRNA levels of ATCA2, COL1A1, COL1A2, and COL3A1 detected using qPCR; F protein levels of α-SMA, collagen I, collagen III, and GAPDH detected using western blotting; and the corresponding quantitative analysis and inter-group comparison are shown. Data are presented as mean ± SD; *P < 0.05 and **P < 0.01