Table 1 Plasmids and Escherichia coli strains used in this study
From: Biosynthesis of ethyl caffeate via caffeoyl-CoA acyltransferase expression in Escherichia coli
Plasmids and E. coli strains | Relevant properties or genetic markers | Source |
|---|---|---|
Plasmids | ||
pCDFDuet | CDF ori, Smr | Novagen |
pETDuet | F1 ori, Ampr | Novagen |
pACYCDuet | P15A ori, Cmr | Novagen |
pC-OS4CL | pCDFDuet carrying 4CL from Oryza sativa | This study |
pC-OS4CL-AtCAT1 | pCDFDuet carrying 4CL from Oryza sativa and CAT from Arabidopsis thaliana | This study |
pC-OS4CL-AtCAT2 | pCDFDuet carrying 4CL from Oryza sativa and CAT from Arabidopsis thaliana | This study |
pE-SeTAL-HpaBC | pETDuet carrying TAL from Saccharothrix espanaensis and HpaBC from Escherichia coli | This study |
pA-aroG-tyrA | pACYCDuet carrying aroG and tyrA from E. coli | [21] |
pA-aroGf-tyrAf | pACYCDuet carrying aroGf and tyrAf from E. coli | [21] |
pA-aroGf-ppsA-tktA-tyrAf | pACYCDuet carrying aroGf, ppsA, tktA, and tyrAf from E. coli | [21] |
pA-aroL-aroGf-ppsA-tktA-tyrAf | pACYCDuet carrying aroL, aroGf, ppsA, tktA, and tyrAf from E. coli | [30] |
pA-aroL-aroE-aroD-aroB-aroGf-ppsA-tktA-tyrAf | pACYCDuet carrying aroL, aroE, aroD, aroB, aroGf, ppsA, tktA, and tyrAf from E. coli | [28] |
pA-aroC-aroA-aroL-aroE-aroD-aroB-aroGf-ppsA-tktA-tyrAf | pACYCDuet carrying aroC, aroA, aroL, aroE, aroD, aroB, aroGf, ppsA, tktA, and tyrAf from E. coli | [31] |
Strains | ||
BL21 (DE3) | F- ompT hsdSB(rB- mB-) gal dcm lon (DE3) | Novagen |
BT | BL21(DE3) FRT-ΔtyrR::FRT-kanR -FRT | [21] |
BTP | BL21(DE3) ΔtyrR::FRT- ΔPheA::FRT-kanR -FRT | [21] |
B-EC1 | BL21 harboring pC-OS4CL-AtCAT1 | This study |
B-EC2 | BL21 harboring pC-OS4CL-AtCAT2 | This study |
B-EC3 | BL21 harboring pC-OS4CL-AtCAT1 and pE-SeTAL-HpaBC | This study |
B-EC4 | BL21 harboring pC-OS4CL-AtCAT1, pE-SeTAL-HpaBC, and pA-tyrA-aroG | This study |
B-EC5 | BL21 harboring pC-OS4CL-AtCAT1, pE-SeTAL-HpaBC, and pA-tyrAf-aroGf | This study |
B-EC6 | BL21 harboring pC-OS4CL-AtCAT1, pE-SeTAL-HpaBC, and pA-aroGf-ppsA-tktA-tyrAf | This study |
B-EC7 | BL21 harboring pC-OS4CL-AtCAT1, pE-SeTAL-HpaBC, and pA-aroL-aroGf-ppsA-tktA-tyrAf | This study |
B-EC8 | BL21 harboring pC-OS4CL-AtCAT1, pE-SeTAL-HpaBC, and pA-aroL-aroE-aroD-aroB-aroGf-ppsA-tktA-tyrAf | This study |
B-EC9 | BL21 harboring pC-OS4CL-AtCAT1, pE-SeTAL-HpaBC, and pA-tyrAf-aroC-aroA-aroL-aroE-aroD-aroB-aroGf-ppsA-tktA | This study |
BT-EC7 | BT harboring pC-OS4CL-AtCAT1, pE-SeTAL-HpaBC, and pA-aroL-aroGf-ppsA-tktA-tyrAf | This study |
BTP-EC7 | BTP harboring pC-OS4CL-AtCAT1, pE-SeTAL-HpaBC, and pA-aroL-aroGf-ppsA-tktA-tyrAf | This study |