Fig. 4

Increased expression of apoptotic marker proteins in CB01-treated cells. U87 and HeLa cells were cultured in 60 mm plates with 0 or 40 nM CB01. After 48 h, cell lysates were prepared and analyzed via western blotting using antibodies against apoptotic marker proteins, such as cleaved caspase-3, cytochrome c, and Bax. β-Actin was used as the internal loading control. By loading a quantified amount of protein in the same amount, the expression of three key apoptosis enzyme proteins was confirmed in the presence or absence of CB01. In addition, it was the same amount as the amount of protein used to confirm the expression of β-actin