Fig. 5

Suppression of CB01-induced caspase-3 activity by the caspase-3 inhibitor Z-VAD-FMK. U87 and HeLa cells were co-treated with 50 µM Z-VAD-FMK and 40 nM CB01 or 5 µM CPT for 48 h. Then, cell lysates were prepared, and their total protein concentrations were quantitated using the Bradford Assay. The caspase-3 activity in each lysate was assessed using a fluorescent assay kit