Fig. 3

HiTrap Q (A) and Sephacryl S-200 (B) chromatograms of Est15L. The blue, red, and black lines of the HiTrap Q chromatogram indicate the absorbance at 280 nm (A.U.), conductivity (dS/cm), and esterase activity (U/mL), respectively. The size exclusion chromatography by Sephacryl S-200 was performed twice, and pools from the 2nd experiment were used for SDS-PAGE