Fig. 6From: Biochemical characterization of a family IV esterase with R-form enantioselectivity from a compost metagenomic librarySubstrate specificity (A) and Lineweaver–Burk plot (B) of Est15L. For substrate specificity, p-NP esters were used for esterase assay at 1 mM. For the cholinesterase assay, 0.5 mM substrates were used. Kinetic study was performed using 0.05, 0.1, 0.2, 0.5, and 1.0 mM of C4Back to article page