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Applied Biological Chemistry

Table 3 Comparison of Est15 and other family IV esterases

From: Biochemical characterization of a family IV esterase with R-form enantioselectivity from a compost metagenomic library

Protein

Accession

Source

Homology (%)

AA

MW (kDa)

Native form*

Opt temp. (°C)

Opt. pH

Preferred pNP esters

Organic solvent effects (%)**

Enantio-selectivity

Ion effect***

Refs.

IPA

MeOH

ACN

Est15L

 

Uncultured bacterium

 

328

34.7

Di

50

9

C4

12.5a

0.9 a

0.3 a

R

(−)Cu2+, (−)Zn2+, (−)Mn2+, (−)Co2+

This study

Est3K

AKG92633

Uncultured bacterium

9.11

299

32.4

 

50

9

C4

87.5a

86.9 a

47.7 a

 

(−)Cu2+

[18]

Est8L

QZA73595

Uncultured bacterium

85.03

314

33.2

Di

40

9

C4

42.68

74.8

33.4

S

(−)Cu2+, (−)Zn2+, (−)Co2+

[24]

EstCS1

AEQ63714

Uncultured bacterium

30.86

309

34.5

 

50

8

C3

115.8 a

90.8 a

101.1a

  

[25]

Rv0045c

CCP42767

Mycobacterium tuberculosis

3.17

298

32.1

 

39

8

C6

     

[35, 36]

EstAM

ACF04196

Uncultured bacterium

12.80

314

34.1

 

40

 

C2

     

[37]

AFEST

WP_010879212

Archaeoglobus fulgidus

30.72

311

35.5

Mono

80

7.1

C6

     

[38, 39]

Cest-2923

CCC79999

Lactobacillus plantarum

4.05

276

30.4

Di, Tetra

30

7

C2

     

[40]

EST2

QGT40748

Alicyclobacillus acidocaldarius

10.39

311

34.4

Mono

70

7.1

C6

     

[41]

EstA1 f

PHS53692

Alteromonas sp.

6.24

379

41.2

 

45

8

C2

102 c

100 c

68 c

  

[42]

Est2L#

QZX52242

Uncultured bacterium

7.22

839

92.5

Di

60

10.0

C2

15.7a

92.7a

47.7a

X

(−)Cu2+, (+)Mg2+, (+)Mn2+, (+)Fe2+

[43]

BFAE

AAC12774

Bacillus subtilis

8.21

372

39.8

Di

  

C4

     

[44]

Est25

AAY45707

Uncultured bacterium

8.53

362

38.3

Di

25

7.0

C4

     

[45]

BaEstB

APW29213

Bjerkandera adusta

5.58

322

34.5

Di

50

7

C2

0.73 a

8.45 a

0.29a

 

(−)Al+, (−)Ni2+ (−)Fe2+, (−)Zn2+

[46]

EstE5

ABI18351

Uncultured bacterium

7.11

297

31.9

Mono

35

9.0

C4

     

[47]

E40

AKF17659

Uncultured bacterium

7.55

297

32.1

Tetra

45

8

C4

    

( +)Na+

[48, 49]

REst1

CBN72524

Rheinheimera sp.

5.91

342

37.5

Mono, Tri

50

8

C4

 

0 a

   

[50]

E69

AUD08548

Erythrobacter seohaensis

6.02

274

29.5

 

60

10.5

C4

0 c

28.8 c

35.0 c

 

(+)Na+

[51]

EstZ

AAM16269

Pseudomonas putida

11.93

318

34.3

 

40

7.5

C2

     

[52]

PestE

BAC06606

Pyrobaculum calidifontis

11.38

313

34.4

Di

90

7

C6

110 b

109 b

117 b

R

 

[53, 54]

Est22

AFB82695

Uncultured bacterium

7.33

344

36.7

Di

40

7.5

C2

40 c

120 c

0 c

 

(−)Zn2+, (−)Cu2+

[55]

Rv1399c

P9WK87

Mycobacterium tuberculosis

10.07

319

33.9

 

45

7

C2

     

[56]

SAestA

AIY29984

Salinispora arenicola

9.96

324

33.8

 

25

9

C4

 

113 e

  

(−)Hg2+, (−)Cu2+, (−)Zn2+

[57]

PMGL2

AMR72657

Uncultured bacterium

6.56

343

36.4

 

45

8.5

C4

 

47.4a

4.2a

 

(+)Na+, (−)Zn2+

[58]

EstE1

AAW62260

Uncultured archaeon

10.28

311

33.8

Di

95

6

C6

     

[59]

SaHSL

AEP27067

Salinisphaera sp.

12.47

316

34.4

 

70

 

C2

75a

   

(0)Na+

[60]

Rv1076

O53424

Mycobacterium tuberculosis

6.89

297

31.7

 

40

8

C4

     

[61]

Est4

CCI69497

Rhodococus sp.

7.66

313

33.2

 

30

7

C4

    

(−)Cu2+, (−)Zn2+, (−)Fe2+, (−)Ag2+, (−)Co2+

[62]

EST3

ALP75907

Parvibaculum sp.

13.57

312

32.8

 

41

6

C5

38.2 d

47.7 d

  

( +)Mn2+, Li2+

[63]

EstE7

ABI18352

Uncultured bacterium

7.22

309

33.0

Di

40

5

C4

     

[64, 65]

Sto-Est

BAB65028

Sulfurisphaera tokodaii

11.05

303

33.8

Di

60

6

C8

     

[66]

EstB28

AFV75078

Oenococcus oeni

6.24

303

34.5

 

40

5

C2

    

(0)Na+

[67]

EstKT4

ADH59412

Uncultured bacterium

7.11

352

38.2

 

40

8.5

C5

    

(−)Mn2+, (−)Zn2+, (−)Ni2+, K + 

[68]

EstKT7

ADH59413

Uncultured bacterium

6.67

316

34

 

35

8

C5

    

(−)Cu2+, (−)Zn2+, (−)Ni2+, Na + 

EstKT9

ADH59414

Uncultured bacterium

6.02

372

40.8

 

45

8.5

C5

    

(−)Zn2+

EstAG1

QBH67630

Staphylococcus saprophticus

5.03

300

35.0

 

47.5

7.5

C8

0d

0d

  

(−)Fe3+, (−)Ba2+, (−)Co2+, (−)Cd2+, (−)Mn2+, (−)Ni2+, (−)Ca2+, (−)K+, (−)Mg2+, (−)K+

[69]

DMWf18-543

AUF80930

Uncultured bacterium

7.66

302

32.3

 

40

7

C4

0 c

0 c

0 c

 

(−)Cu2+, (−)Zn2+, (−)Ni2+, (−)Co2+

[70]

DMWf18-558

AUF80945

Uncultured bacterium

7.66

302

32.1

 

40

7

C2

0 c

0 c

0 c

 

(−)Cu2+, (−)N2+-, (−)Co2+, (−)Zn2+, (−)Mn2+

  1. *Mono, monomer; Di, dimer; Tri, trimer; Tetra, tetramer; Hexa, hexamer. ** Organic solvent stability at a concentration of a 30%, b 50%, c 15%, d 10%, and e 25%, *** X means it does not have enantioselectivity, S or R means it has S-form or R-form enantioselectivity, respectively. **** (+) Means it is activated by the ion, (−) means it is inhibited by the ion and (0) means that it showed stability by the ion. # Since Est2L is a fusion type esterase, the esterase domain (422 AA) was used for homology analysis
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