Fig. 5
From: Anti-acne effects of Castanea crenata bur extract and identification of active compound
CBE decreased C. acnes CM-induced NF-κB p65 translocation and IκBα phosphorylation in HEKn. A Immunofluorescence of NF-κB p65 subunit translocation in HEKn cultured in C. acnes CM or C. acnes CBE. The blue and green fluorescence indicates the nucleus and NF-κB p65 subunit, respectively. B After cell lysis, phosphorylated IκBα, IκBα, and GAPDH protein expression were detected by western blots. The protein levels were normalized to that of GAPDH, n = 3