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Fig. 6 | Applied Biological Chemistry

Fig. 6

From: Anti-inflammatory components isolated from Atractylodes macrocephala in LPS-induced RAW264.7 macrophages and BV2 microglial cells

Fig. 6

The effect of 8-epiasterolid on the LPS-induced expression of iNOS and COX-2 protein in RAW264.7 (A) and BV2 cells (B). Lysates were prepared from cells pre-treated with or without the indicated concentrations of 8-epiasterolid for 3 h and then with LPS (1 μg/mL) for 24 h. The level of expression of iNOS and COX-2 proteins was determined by Western blot analysis. Representative blots from three independent experiments are shown

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