Fig. 2

Determination of effective dsRNA concentrations for dipping and spray methods. a, b Synthesized dsRNA_EGFP_5′ was diluted in water to final concentrations of 5, 10, 20 or 40 ng/μL. Diluted dsRNAs were then applied to 1-week-old wild-type Col-0 seedlings by dipping and spray methods, respectively. Using qRT-PCR, the expression level of dsRNA_EGFP_5′ was quantified at 1 day post-treatment (dpt), 2 dpt, 4 dpt or 6 dpt for plants subjected to dsRNA application using the dipping method (a) and spray method (b), respectively