Fig. 2From: Selection of the high efficient sgRNA for CRISPR-Cas9 to edit herbicide related genes, PDS, ALS, and EPSPS in tomatoConstruction map used for expressing CRISPR-Cas9 system transiently. sgRNA and Cas9 are conducted with AtU6 promoter and 35S promoter, respectively. The vector contains Kanr gene for bacterial selection and Hygr gene for plant selection. The ALS2_P sgRNA (the sequences in red) is indicated to present the cloning site of sgRNABack to article page