Fig. 2From: Schizophyllum commune-derived β-glucan improves intestinal health demonstrating protective effects against constipation and common metabolic disordersEffect of β-glucan on mucus thickness and goblet cell proliferation. a The intracellular mucus of the goblet cells is stained using periodic acid–Schiff (PAS); b however, PAS could not stain the acidic mucus, which is stained by Alcian blue instead; c mucosa layer thickness was measured by applying ImageJ software to analyze sectioned intestine images. Statistically significant results are labeled as *P < 0.05; **P < 0.01; ***P < 0.001Back to article page