Fig. 2

SM reduced the induced inflammation in macrophages and CD4 cells. A, B SM regulates cytokines of neuroinflammation in EAE mice; IL-6, TNF-α from macrophage of glial cells. C Mononuclear cell count in CNS using hematological examination gives a total count of leukocytes in the EAE mice. D Cytokine estimation of IL-17, IFN-γ and IL-17 in CD3−CD28 activated CD4 cells were treated with SM in a concentration of 10, 25 and 50 μM. E GM-CSF cytokine were estimated in stimulated macrophages. CD3−CD28 activated CD4 cells were treated with SM in a concentration of 10, 25 and 50 μM. F mRNA expression of NFKB, MAPK6 and STAT3 were quantified using quantitative real time PCR and G immunoblot of NFKB, MAPK6 and STAT3 protein were estimated and β actin used as internal control. All data were expressed as mean ± SEM and the differences between variables were analyzed using student T test. The differences were considered significant when the P values were less than 0.05