Fig. 5

Effect of SM on oxidative mediated neuropathological markers in EAE mice (A–D). Different markers were investigated in spinal tissue homogenates. A Malondialdehyde is an marker for inflammatory response as well as neutophile infilterations. This was raised in EAE mice compared with SM treated EAE mice. B SOD/CAT ratio was examined the primary oxidative stress of EAE and SM treated EAE mice. C Glutathione antioxidant was examined for the evaluation of total glutathione in the EAE mice and SM treated mice. D Acetylcholine esterase activity was evaluated the neuro communication interactions. It was secreted more in myelinated EAE disease conditions. E, F Immunohisto examination of iNOS in SM treated EAE mice and naïve EAE mice. All data were expressed as mean ± SEM and the differences between variables were analyzed using student T test. The differences were considered significant when the P values were less than 0.05