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Applied Biological Chemistry
Fig. 5 | Applied Biological Chemistry

Fig. 5

From: Glycyrrhiza uralensis attenuates TNF-α-induced muscle atrophy in myoblast cells through the Nrf2 and MAFbx signaling cascades

Fig. 5

Glycyrrhiza uralensis increased Nrf2 and heme oxygenase-1 protein levels in C2C12 cells. Cells were incubated with GU (10, 50, and 100 μg/mL) for 30 min; total and nuclear proteins were then harvested from the cells. Cell lysates were prepared for western blot analysis. Nuclear Nrf2 expression in C2C12 cells was normalized to lamin B, and the expression levels of other proteins were normalized to β-actin. Data are presented as the means ± standard error of the means (SEMs) of three independent experiments

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