Fig. 2

IC261 inhibits epithelial mesenchymal transition marker protein expression stimulated by TGF-β1 in A549 cells. A 6-well plate containing serum-free culture medium was seeded with A549 cells and incubated for 6 h. After 4 h of treatment with IC261 (50 and 75 µM), the cells were stimulated with TGF-β1 for 48 h. Specific antibodies were used for immunoblotting of the cell lysates. A Expression of N-cad, Vim, β-cat, and E-cad. B Protein expression level was determined using ImageJ software and normalized against the expression of GAPDH. The data are expressed as the mean ± SD (n = 3). ^#P < 0.0001 in comparison to the control and ^***P < 0.0001 compared with the TGF-β1 group