Fig. 4.

5-MTP pretreatment alleviated LPS-induced cardiac inflammation by inhibiting the activation of NF-κB signaling pathway in mice. A–E, western blot analysis was applied to detecting the protein levels of inflammatory cytokines (IL-1β, IL-6 and TNF-α), NLRP3 inflammasome and anti-inflammatory cytokine (IL-10) in mouse heart tissues (n = 8). F, the change of protein expression level of p-NF-κB was investigated by western blot assay (n = 8). *p < 0.05, **p < 0.01. Data represent the mean ± SD of at least 3 times of separate experiments