Fig. 2
From: CircCEP85 upregulates IGF1 expression to promote breast cancer progression via sponging miR-1193
Knockdown of circCEP85 inhibited proliferation, invasion, angiogenesis, stemness and induced apoptosis of BC cells in vitro. A Relative expression of circCEP85 was detected by qRT-PCR in BT-549 and MDA-MB-231 cells transfected with sh-NC, sh-circCEP85#1, sh-circCEP85#2, or sh-circCEP85#3; B–H BT-549 and MDA-MB-231 cells were transfected with sh-NC or sh-circCEP85#1. B, C CCK-8 assay and EDU assay were performed to measure cell proliferation; D Flow cytometry analysis was conducted to evaluate cell apoptosis rate; E Transwell assay was used to detect cell invasion ability; F Angiogenesis capability was assessed by tube formation assay; G Sphere formation assay was used to detect cell stemness; H Western blot assay was employed to detect the levels of proliferation-related (PCNA), apoptosis-related (cleaved caspase-3), angiogenesis-related (VEGFA) and stemness-related (Naong) protein. **P < 0.01, ***P < 0.001