Fig. 3
From: CircCEP85 upregulates IGF1 expression to promote breast cancer progression via sponging miR-1193
CircCEP85 functioned as a sponge for miR-1193. A The location of circCEP85 was tested by cytoplasmic and nuclear RNA analysis assay; B Prediction of downstream target genes for circCEP85 binding using bioinformatics websites; C Relative expression of miR-1193 in BC tissues and adjacent non-cancer tissues was detected by qRT-PCR; D The correlation between circCEP85 and miR-1193 in BC tissues was analyzed by Pearson correlation analysis; E Relative expression of miR-1193 in BC cells was detected; F The overexpression efficiency of miR-1193 mimic was detected by qRT-PCR; G Schematic diagram of binding sites between circCEP85 and miR-1193; H, I Dual-luciferase reporter assay was performed to analyze the target binding relationship between circCEP85 and miR-1193; J RNA pull down assay was performed to assess the relationship between circCEP85 and miR-1193; K Enrichment levels of circCEP85 and miR-1193 were measured by RIP assay. **P < 0.01, ***P < 0.001