Fig. 5

IGF1 was a direct target of miR-1193. A The downstream target genes of miR-1193 were predicted using bioinformatics websites; B The expression of downstream target genes in BC cells treated with miR-1193 mimics was detected by qRT-PCR; C Schematic diagram of binding sites between IGF1 and miR-1193; D The relative luciferase activities of the reporter were detected in BC cells; E Western blot was conducted to detect the protein level of IGF1 in BC cells after the transfection with miR-1193 mimics; F Relative expression of IGF1 mRNA in BC tissues and adjacent non-cancer tissues was detected by qRT-PCR; G, H IHC and western blot assays were used to detect IGF1 expression in BC tissues and adjacent non-cancer tissues; I The protein level of IGF1 in BC cells was assessed by western blot. ***P < 0.001