Fig. 6
From: CircCEP85 upregulates IGF1 expression to promote breast cancer progression via sponging miR-1193
CircCEP85 promoted BC progression via regulating miR-1193/IGF1 axis. A The knockdown efficiency of miR-1193 was detected by qRT-PCR; B The overexpression efficiency of IGF1 was assessed by western blot analysis; C–I BC cells were treated with sh-NC, sh-circCEP85#1, sh-circCEP85#1 + anti-NC, sh-circCEP85#1 + anti-miR-1193, sh-circCEP85#1 + vector and sh-circCEP85#1 + IGF1, respectively; Cell proliferation was detected by CCK8 assay (C) and EDU assay (D); Cell apoptotic rate was assessed by flow cytometry analysis (E); Cell invasion ability was measured by transwell assay (F); Angiogenesis capability was evaluated by tube formation assay (G); Cell stemness was estimated by sphere formation assay (H); I The levels of PCNA, cleaved caspase 3, VEGFA and Naong proteins were detected by western blot analysis. **P < 0.01, ***P < 0.001