Parameter | Analytical condition |
---|---|
Equipment | Waters 1525 binary HPLC MA 01757 (USA) Waters 2424 ELSD MA 01757 (USA) |
Column | YMC pack pro C18 column (25 cm × 4.6 mm, 5 μm) |
Oven temperature | 30 °C |
Injection volume | 10 μL |
Mobile phase | Gradient (see Table 2) |
Flow rate | 1.1 mL/min |
ELSD | 90 °C; N2 gas, 40 psi |