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Applied Biological Chemistry
Fig. 3 | Applied Biological Chemistry

Fig. 3

From: Depressing hsa_circ_0058092 functions an integrated anti-proliferation and anti-motility role in gastric cancer partially through targeting miR-1294/SIX1 axis

Fig. 3

Physical interaction between hsa_circ_0058092 and miR-1294. A Venn diagram showed the number of miRNAs that were predicted as targets for hsa_circ_0058092 in starBase, circBank and CircInteractome online software. B–D qPCR examined relative expression of (B) miR-140-3p, miR-149-5p, miR-223-3p, miR-324-5p, miR-486-5p, miR-889-3p, miR-1294, miR-520 h, and miR-520 g-3p in 6 pairs of randomly selected Normal and Tumor tissues, and (C, D) miR-140-3p, miR-149-5p, miR-324-5p, miR-486-5p, and miR-1294 in AGS and HGC-27 cells transfected with si-hsa_circ_0058092 (#2) or si-NC. (E) Schematic diagram showed the wild-type and mutations of the predicted miR-1294-binding sites in hsa_circ_0058092 WT and MUT. (F, G) Dual-luciferase reporter assay determined relative luciferase activity of hsa_circ_0058092 WT and MUT reporter vectors in response to miR-1294 mimic (miR-1294) or miR-NC mimic (miR-NC) in AGS and HGC-27 cells. H, I RIP assay testified relative RNA enrichment of hsa_circ_0058092 and miR-1294 in the Input, Anti-IgG group and Anti-Ago2 group in cell lysate of AGS and HGC-27 cells. J, K qPCR examined relative expression of miR-1294 in J 41 pairs of Normal and Tumor tissues, and K GES-1, AGS and HGC-27 cells. L Pearson’s correlation coefficient was determined between relative hsa_circ_0058092 and miR-1294 levels in 41 Tumor tissues. ***P < 0.001

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