Fig. 4

Deficiency of miR-515-5p attenuated circTRRAP knockdown-mediated effects on PCa cell malignancy and glycolysis. A miR-515-5p abundance was measured in PCa cells transfected with si-NC, si-circTRRAP#1, si-circTRRAP#1 + inhibitor-NC, or si-circTRRAP#1 + miR-515-5p inhibitor by qRT-PCR. B–F Cell viability, proliferation, migration and invasion were detected in the above cells by MTT, EdU and transwell assays. G PCNA, MMP2, and MMP9 protein levels were detected in the above cells by western blot. H and I The glucose uptake and lactate production of the above cells were measured. *P < 0.05