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Applied Biological Chemistry
Fig. 3 | Applied Biological Chemistry

Fig. 3

From: Production of isoquercitrin from quercetin by biotransformation using Bascillus sp. CSQ10 isolated from Camellia sinensis cultivation soils

Fig. 3

Effect of biotransformation medium on isoquercitrin production by Bacillus sp. CSQ10. A Isoquercitrin production titers according to biotransformation medium; B standard quercetin; C standard isoquercitrin; D HPLC analysis of reaction products of YPD medium biotransformation; E MS spectra of P2 in positive ion mode; F MS spectra negative ion mode of P2. Bacillus sp. CSQ10 cell density was adjusted to OD600 = 0.6, with 2 mL of each medium and 200 µM quercetin. The resulting culture was biotransformed at 30 °C for 12 h, with shaking at 200 rpm. The reaction products were extracted with two volumes of ethyl acetate and analyzed using HPLC. Error bars indicate mean values ± from three independent experiments

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