Fig. 5

The regulation of circ-NT5C2 on OS cells malignant behaviors in vitro was relieved by miR-497-5p. A–B The overexpression and knockdown transfection efficient of miR-488-3p mimic and inhibitor was confirmed by qRT-PCR in SW1353 cells and U2OS cells, respectively. C–R SW1353 cells were transfected with Vector, circ-NT5C2, circ-NT5C2 + miR-NC, circ-NT5C2 + miR-488-3p and U2OS were transfected with si-NC, si-circ-NT5C2#2, si-circ-NT5C2#2 + in-miR-NC, or si-circ-NT5C2#2 + in-miR-488-3p. C–H The cell proliferation was assessed by CCK-8 assay C–D, colony formation assay E–F and EdU assay (G–H). I–J The cell apoptosis of was detected by flow cytometry. K–L Activity Assay Kits were used to test the Caspase3 and Caspase9 activity. M–P The cell migration and invasion were determined by transwell assay. Q–R The protein expression of E-cadherin and N-cadherin was detected by WB. *P < 0.05, **P < 0.01 and ***P < 0.001