Fig. 6

MiR-488-3p directly targeted FZD4. A The binding sites of miR-488-3p and FZD4 3’UTR MT and MUT vector were predicted by TargetScan online software. B–C FZD4 expression was detected by WB in SW1353 cells transfected with miR-NC or miR-488-3p and U2OS cells transfected with in-miR-NC or in-miR-488-3p. D–E The luciferase activity was measured by dual-luciferase reporter assay in SW1353 and U2OS cells transfected with FZD4 3’UTR WT/MUT reporter vector and miR-488-3p or miR-NC. F–I FZD4 expression was examined by qRT-PCR and WB in OS tissues and cell samples. **P < 0.01 and ***P < 0.001