Fig. 2

GB extract reduces ceramide generation. MIN6 cells were treated with GB extract (200 µg/ml) for 24 h, followed by 0.5 mM PAL for another 24 h. A Relative mRNA levels of CerS, B representative western blots of CerS, and C total ceramide levels were examined (n = 3). D Relative CerS mRNA levels in MIN6 cells (n = 3). Values are expressed as mean ± SEM compared to control group; *P < 0.05, **P < 0.01 and compared to PAL-treated group; #P < 0.05, ##P < 0.01, ###P < 0.001. All experiments were performed in triplicate. GB Gryllus bimaculatus; PAL palmitate; CerS ceramide synthase, Con control (untreated)