Fig. 3From: Sequence optimization and multiple gene-targeting improve the inhibitory efficacy of exogenous double-stranded RNA against pepper mottle virus in Nicotiana benthamianaGFP transcript levels measured by quantitative PCR. Levels of GFP transcript tagged to the PepMoV genome were measured by quantitative PCR in the local leaves of dsRNA-treated plants at 8 dpi. R.luc indicates the plants treated with dsRNA containing partial sequences (500-bp-long) of Renilla luciferase. The relative expression level of each sample was measured and compared with non-dsRNA-treated plants. The data represents the average of three independent experiments and error bars represent SEM. Significance is determined by Student’s t-test, *: P < 0.05, **: P < 0.01Back to article page