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Applied Biological Chemistry
Fig. 7 | Applied Biological Chemistry

Fig. 7

From: Chelidonic acid ameliorates atopic dermatitis symptoms through suppression the inflammatory mediators in in vivo and in vitro

Fig. 7

Effect of CA on NF-κB activation in AD-like skin lesion and LPS-stimulated mouse peritoneal macrophages. A The nuclear extract of skin tissue was isolated by Nuclear Extraction Reagents. The levels of NF-κB p65 in nucleus were evaluated by western blot analysis. B The relative expression level of NF-κB p65 was measured using an image analyzer. C Cells (6 × 106) were pretreated for 1 h with CA and then treated for 1 h with LPS. The levels of NF-κB p65 in cell nucleus were evaluated by Western blot analysis. D The relative expression level of NF-κB p65 was measured using an image analyzer. The data represents the mean ± S.D. of three independent experiments (#p < 0.05 vs. control group, *p < 0.05 vs. DNCB-treated group, **p < 0.05 vs. LPS-stimulated cells)

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