Fig. 3

BLM increases α-SMA and NOX2 expression while decreasing E-cadherin in A549. A A549 cells were treated with BLM with concentrations of 5, 10, 20, 40, 80 μg/ml for 48 h. The graph shows the relatively quantified value of western blot results if that of the control group against actin was 1. B We treated BLM of 10, 20, 40, 60, 80, 100 μg/ml for 48 h and replaced the media with DCF-DA (10 μM) dissolved PBS. The images are observed under the 100 × fluorescence microscope. C After treating BLM (20 μg/ml) for 48 h, the cellular levels of α-SMA (red), E-cadherin (green) and NOX2 (green) were observed by immunofluorescence and the images are shown compared to the control group