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Fig. 4 | Applied Biological Chemistry

Fig. 4

From: Cherry fruit anthocyanins cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside protect against blue light-induced cytotoxicity in HaCaT cells

Fig. 4

Effects of C3OG and C3OR on the expression of inflammatory cytokines induced by blue light. HaCaT cells in six-well plates were exposed to blue light (20,000 lx) for 1 h. Cells were pretreated with or without NAC (10 mM), FAK-i (1 µM), or NAC + FAK-i for 1 h or different concentrations (10, 50, 100, or 200 µM) of C3OG or C3OR for 24 h prior to blue light exposure. After 24 h, supernatants were collected, and the amounts of TNF-α (A), IL-6 (B), and IL-8 (C) were determined by ELISA (n = 3, ±SD). ** p < 0.01 and *** p < 0.001 vs. blue light (20,000 lx)

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