Fig. 8
APDT effects against MRSA and C. acnes with LFE on micropig skin. Overnight culture of MRSA or C. acnes was incubated with or without LFE (20 µg/mL; 30 min incubation) and spread on the micropig skin at 5 µL/cm2. Ampicillin (100 µg/mL) was used as a positive control. The coated skin was then illuminated under red light at 20 W/m2 for 30 min. The treated skin was imprinted on BHI agar to illustrate the inhibition of C. acnes or MRSA on the surface of the skin by APDT. Representative images of MRSA growth on LB plates (A) and C. acnes growth on BHI plates (C) were recorded after 48-hour incubation. The differently treated skins were also washed with phosphate-buffered saline using a swab. The total content of bacteria on the swab of each treatment was plated on LB or BHI agar plates. B, D) CFU formation after 48 h was quantified and represented in the graph as a log (CFU/cm2). The data are reported as mean ± standard error (n = 2) and representative of at least two independences. ns indicates no statistical significance relative to the control group. Significant differences are expressed with * for p < 0.05, ** for p < 0.01, and *** for p < 0.001 relative to the vehicle control. The log reductions compared with vehicle control are presented in each column