Fig. 3
From: Analysis of protein binding characteristics among Arabidopsis BBX protein family
Protein–protein interactions among 32 B-box proteins. A. Analysis of 1024 protein–protein interactions was performed using a 96-well format system. BD and AD fusion constructs were individually transformed into yeast. To determine and distinguish interaction strengths, yeast cells were spotted and grown on SD agar media (1) lacking T, L, and H in the presence of 0.1–0.5 mM 3-AT, (2) lacking T, L, and H in the presence of 1–5 mM 3-AT, (3) lacking T, L, and H in the presence of 10 mM 3AT, or (4) lacking T, L, A, and H. Positive pPC97-Fos/pPC86-Jun and pDBLeu/pEXP-AD502 combinations were used as positive and negative interaction controls. B. BBX family dimerization map. Strong, moderate, and weak interactions are indicated by black, dark gray, and light gray, respectively