Simultaneous quantification of ferrous gluconate and calcium gluconate in foods using liquid chromatography–tandem mass spectrometry (LC–MS/MS)

Applied Biological Chemistry

Table 1 MRM transition and optimized parameters of LC–MS/MS for FG and CG

Compound	Ion mode (ESI+/−)	Molecular weight(g/mol)	Precursor ion (m/z)	Product ion (m/z)	Collision energy (eV)	Cone voltage (V)	Retention time (min)
Ferrous gluconate	+	446.1	447	411^a	22	28	0.79
				293	14	28	0.79
				205	36	28	0.79
				191	12	28	0.79
				175	14	28	0.79
Calcium gluconate	+	430.4	431	333^a	24	28	0.73
				317	15	28	0.73
				235	28	28	0.73
				175	34	28	0.73
				159	34	28	0.73