Table 3 Results of accuracy and precision for FG and CG using simultaneous LC–MS/MS method
Conc. (μg/mL) | Ferrous gluconate | |||||
|---|---|---|---|---|---|---|
Intra-day test (n = 5) | Inter-day test (n = 3) | |||||
Measured concentration (μg/mL) | Accuracy (recovery%) | Precision (RSD%) | Measured concentration (μg/mL) | Accuracy (Recovery%) | Precision (RSD%) | |
50 | 54.3 ± 0.8 | 108.6 | 1.5 | 54.9 ± 1.3 | 109.7 | 2.4 |
100 | 104.4 ± 0.4 | 104.4 | 0.4 | 107.8 ± 0.4 | 107.8 | 0.3 |
200 | 207.8 ± 4.3 | 103.9 | 2.1 | 204.2 ± 0.9 | 102.1 | 0.4 |
300 | 304.0 ± 4.9 | 101.3 | 1.6 | 294.8 ± 2.0 | 98.3 | 0.7 |
400 | 390.8 ± 8.7 | 97.7 | 2.2 | 409.6 ± 5.5 | 102.4 | 1.3 |
Conc. (μg/mL) | Calcium gluconate | |||||
|---|---|---|---|---|---|---|
Intra-day test (n = 5) | Inter-day test (n = 3) | |||||
Measured concentration (μg/mL) | Accuracy (recovery%) | Precision (RSD%) | Measured concentration (μg/mL) | Accuracy (recovery%) | Precision (RSD%) | |
50 | 52.5 ± 3.4 | 105.0 | 6.4 | 55.4 ± 1.0 | 110.8 | 1.8 |
100 | 100.1 ± 3.0 | 100.1 | 3.0 | 94.3 ± 1.3 | 94.3 | 1.4 |
200 | 206.4 ± 4.1 | 103.2 | 2.0 | 202.4 ± 1.5 | 101.2 | 0.8 |
300 | 303.0 ± 3.3 | 101.0 | 1.1 | 311.4 ± 4.3 | 103.8 | 1.4 |
400 | 393.2 ± 6.2 | 98.3 | 1.6 | 403.1 ± 4.5 | 100.8 | 1.1 |