Establishment of analysis method for the quantification of residues of halquinol and its metabolites in livestock and fishery products using liquid chromatography–tandem mass spectrometry

Lee, Tae Ho; Park, Sun Young; Kim, Ji Young; Choi, Jang-Duck; Moon, Guiim

doi:10.1186/s13765-023-00793-3

Applied Biological Chemistry

Table 2 Comparison of linearity, accuracy, and precision of analysis method by extraction solution(n = 5)

From: Establishment of analysis method for the quantification of residues of halquinol and its metabolites in livestock and fishery products using liquid chromatography–tandem mass spectrometry

Sample	Compound	80% MeCN containing 100 ppm of Na₂-EDTA				80% MeCN
Sample	Compound	R²	Spiking levels (μg kg⁻¹)	Average recovery (%)	CV (%)	R²	Spiking levels (μg kg⁻¹)	Average recovery (%)	CV (%)
Beef	5-CHQ	0.9989	5	96.73	8.15	0.9814	5	120.37	12.72
			10	97.18	3.73		10	110.37	19.44
			20	100.41	3.86		20	81.92	16.39
	5,7-DCHQ	0.9989	5	103.00	7.32	0.9923	5	110.78	6.39
			10	105.77	7.90		10	91.62	4.83
			20	111.14	9.29		20	80.17	6.14
	5-CHQG	0.9972	5	115.92	4.57	0.9998	5	94.49	12.42
			10	92.50	9.56		10	83.61	5.67
			20	76.40	5.40		20	85.80	9.31
	5,7-DCHQG	0.9968	5	118.65	5.34	0.9988	5	104.63	12.05
			10	96.32	6.56		10	92.02	7.50
			20	77.70	5.31		20	90.41	8.48
Pork muscle	5-CHQ	0.9981	20	94.80	7.75	0.9521	20	101.60	12.34
			40	103.40	4.24		40	110.40	39.60
			80	106.56	3.05		80	64.32	18.10
	5,7-DCHQ	0.9996	20	106.86	4.12	0.9867	20	111.48	8.33
			40	104.54	4.28		40	104.32	14.59
			80	100.42	7.25		80	85.30	5.36
	5-CHQG	0.9996	20	97.77	16.03	0.9990	20	92.16	20.63
			40	87.18	14.22		40	81.68	11.86
			80	90.48	13.52		80	102.51	14.30
	5,7-DCHQG	0.9991	20	94.14	15.62	0.9941	20	81.94	21.87
			40	87.90	14.20		40	80.84	9.35
			80	93.09	12.10		80	99.52	11.73
Chicken	5-CHQ	0.9998	5	95.80	9.05	0.9125	5	67.06	26.98
			10	99.68	5.38		10	69.86	84.03
			20	97.94	3.79		20	131.63	28.13
	5,7-DCHQ	0.9988	5	95.99	8.57	0.9867	5	70.33	16.33
			10	99.78	6.47		10	77.80	42.85
			20	98.56	3.32		20	123.57	18.00
	5-CHQG	0.9969	5	112.92	11.11	0.9975	5	104.62	8.05
			10	99.08	3.24		10	95.51	11.56
			20	78.44	3.58		20	103.75	10.34
	5,7-DCHQG	0.9975	5	119.34	2.74	0.9982	5	99.57	5.58
			10	98.98	3.48		10	91.78	8.66
			20	77.32	2.70		20	99.28	10.77
Egg	5-CHQ	0.9996	5	97.60	8.93	0.9716	5	152.67	24.82
			10	106.12	2.99		10	153.92	10.29
			20	96.89	6.68		20	102.44	21.39
	5,7-DCHQ	0.9995	5	104.60	2.95	0.9987	5	101.29	5.91
			10	104.47	5.50		10	89.44	3.22
			20	105.98	2.77		20	78.68	7.30
	5-CHQG	0.9982	5	90.37	27.98	0.9997	5	114.49	4.77
			10	103.11	16.11		10	110.98	5.92
			20	101.77	13.63		20	90.21	6.76
	5,7-DCHQG	0.9971	5	96.90	28.66	0.9992	5	112.84	2.74
			10	99.98	12.05		10	114.72	3.95
			20	99.19	9.80		20	94.39	7.63
Milk	5-CHQ	0.9995	5	91.30	4.95	0.9902	5	78.31	29.95
			10	95.59	4.89		10	42.82	23.69
			20	98.57	3.53		20	46.66	39.86
	5,7-DCHQ	0.9987	5	105.81	8.50	0.9921	5	96.57	32.62
			10	106.06	10.55		10	63.27	18.34
			20	107.00	5.15		20	68.18	16.72
	5-CHQG	0.9944	5	120.22	28.07	0.9881	5	149.63	30.45
			10	90.45	27.83		10	129.39	11.99
			20	109.82	25.48		20	200.95	21.51
	5,7-DCHQG	0.9987	5	88.65	27.17	0.9919	5	106.48	49.77
			10	81.65	24.94		10	128.60	8.86
			20	102.48	25.75		20	169.59	25.00

Back to article page