Establishment of analysis method for the quantification of residues of halquinol and its metabolites in livestock and fishery products using liquid chromatography–tandem mass spectrometry

Lee, Tae Ho; Park, Sun Young; Kim, Ji Young; Choi, Jang-Duck; Moon, Guiim

doi:10.1186/s13765-023-00793-3

Applied Biological Chemistry

Table 5 Inter-lab validation results of the accuracy and precision

From: Establishment of analysis method for the quantification of residues of halquinol and its metabolites in livestock and fishery products using liquid chromatography–tandem mass spectrometry

Sample	Compound	Spiking levels (μg kg⁻¹)	Average recovery (%)				CV (%)
Sample	Compound	Spiking levels (μg kg⁻¹)	Institution A^a	Institution B^b	Institution C^c	Total	CV (%)
Beef	5-CHQ	5	96.73	92.73	115.40	101.62	7.12
		10	97.18	88.80	114.13	100.04	5.51
		20	100.41	88.83	102.69	97.31	6.95
	5,7-DCHQ	5	103.00	97.67	118.87	106.51	6.36
		10	105.77	98.03	109.33	104.38	7.52
		20	111.14	98.02	102.11	103.76	9.84
	5-CHQG	5	115.92	99.20	119.93	111.68	9.33
		10	92.50	97.33	113.43	101.09	10.29
		20	76.40	87.85	103.03	89.09	10.15
	5,7-DCHQG	5	118.65	101.93	119.60	113.39	8.91
		10	96.32	95.47	111.33	101.04	7.08
		20	77.70	84.73	102.64	88.36	7.78
Pork muscle	5-CHQ	20	94.80	100.23	106.13	100.39	6.66
		40	103.40	98.08	103.03	101.50	4.30
		80	106.56	93.43	99.99	99.99	7.36
	5,7-DCHQ	20	106.86	102.30	118.67	109.28	4.20
		40	104.54	96.00	109.27	103.27	5.55
		80	100.42	101.35	99.31	100.36	5.74
	5-CHQG	20	97.77	86.40	106.13	96.77	14.47
		40	87.18	107.93	103.03	99.38	15.06
		80	90.48	117.61	99.99	102.69	18.81
	5,7-DCHQG	20	94.14	87.25	118.20	99.86	13.02
		40	87.90	108.65	109.10	101.88	15.02
		80	93.09	117.55	99.87	103.50	16.24
Pork fat	5-CHQ	5	101.13	105.87	119.07	108.69	4.39
		10	102.16	104.13	114.73	107.01	4.86
		20	98.71	95.57	104.98	99.75	5.68
	5,7-DCHQ	5	107.57	99.53	108.87	105.32	8.98
		10	94.77	104.97	104.07	101.27	7.27
		20	95.70	98.70	104.33	99.58	6.97
	5-CHQG	5	103.86	107.47	115.00	108.78	7.62
		10	94.68	103.90	106.27	101.62	7.03
		20	85.09	98.75	106.41	96.75	7.96
	5,7-DCHQG	5	83.63	106.80	116.47	102.30	14.41
		10	81.62	102.57	109.13	97.77	14.14
		20	76.61	98.82	105.98	93.80	16.60
Chicken	5-CHQ	5	95.80	101.60	107.80	101.73	7.38
		10	99.68	104.53	106.90	103.70	5.39
		20	97.94	112.78	103.89	104.87	7.94
	5,7-DCHQ	5	95.99	106.60	118.80	107.13	8.40
		10	99.78	102.57	109.23	103.86	5.10
		20	98.56	107.15	102.02	102.58	5.12
	5-CHQG	5	112.92	93.87	105.73	104.17	13.24
		10	99.08	82.87	104.77	95.57	10.33
		20	78.44	93.37	101.65	91.15	12.59
	5,7-DCHQG	5	119.34	96.53	113.33	109.73	10.93
		10	98.98	79.30	104.93	94.40	11.84
		20	77.32	87.32	102.55	89.06	10.59
Egg	5-CHQ	5	97.60	102.47	107.27	102.45	7.14
		10	106.12	98.80	113.07	106.00	4.75
		20	96.89	98.77	106.72	100.79	6.28
	5,7-DCHQ	5	104.60	106.73	109.33	106.89	2.52
		10	104.47	101.50	110.60	105.52	4.53
		20	105.98	98.15	108.95	104.36	4.62
	5-CHQG	5	90.37	103.60	116.93	103.63	21.32
		10	103.11	100.13	117.80	107.01	12.41
		20	101.77	99.90	109.39	103.69	10.43
	5,7-DCHQG	5	96.90	104.67	108.40	103.32	21.45
		10	99.98	98.80	113.60	104.13	9.35
		20	99.19	98.65	112.49	103.44	7.48
Milk	5-CHQ	5	91.30	103.40	106.47	100.39	8.07
		10	95.59	105.50	100.57	100.55	6.30
		20	98.57	101.17	94.90	98.21	5.33
	5,7-DCHQ	5	105.81	99.67	102.40	102.63	7.50
		10	106.06	103.10	95.50	101.55	8.33
		20	107.00	108.57	96.43	104.00	4.08
	5-CHQG	5	120.22	105.20	106.47	110.63	23.27
		10	90.45	100.93	100.57	97.32	20.97
		20	109.82	101.32	94.90	102.01	20.28
	5,7-DCHQG	5	88.65	98.13	91.40	92.73	20.60
		10	81.65	99.73	90.20	90.53	25.59
		20	102.48	100.42	92.62	98.51	19.65
Flat fish	5-CHQ	5	100.89	81.60	112.27	98.25	10.92
		10	101.96	97.63	108.33	102.64	4.76
		20	97.39	97.62	102.66	99.22	1.08
	5,7-DCHQ	5	85.34	97.47	86.67	89.83	9.77
		10	93.25	101.47	96.97	97.23	4.89
		20	103.92	99.85	108.41	104.06	6.46
	5-CHQG	5	119.36	78.00	85.20	94.19	21.54
		10	109.94	85.80	98.83	98.19	17.04
		20	112.14	94.98	106.99	104.70	13.50
	5,7-DCHQG	5	116.04	83.53	113.73	104.43	22.05
		10	110.29	98.67	104.23	104.40	9.83
		20	108.96	100.32	102.47	103.92	13.15
Eel	5-CHQ	5	97.52	101.93	101.07	100.17	4.32
		10	94.20	109.47	110.70	104.79	8.50
		20	93.98	105.98	111.12	103.69	6.58
	5,7-DCHQ	5	100.82	106.47	117.20	108.16	4.95
		10	96.07	103.17	113.23	104.16	4.91
		20	95.04	105.18	109.93	103.38	6.47
	5-CHQG	5	93.42	100.20	114.93	102.85	20.69
		10	105.79	104.80	117.73	109.44	19.94
		20	112.34	103.12	109.61	108.88	15.49
	5,7-DCHQG	5	92.51	94.60	118.13	93.29	19.41
		10	104.93	100.40	113.20	103.23	18.94
		20	111.81	104.28	110.21	108.99	14.76
Shrimp	5-CHQ	5	102.17	94.73	103.00	99.38	6.86
		10	102.83	100.73	95.67	102.04	3.05
		20	105.69	104.65	98.09	105.30	4.24
	5,7-DCHQ	5	102.45	97.60	96.13	100.63	4.07
		10	107.33	105.00	93.57	106.46	5.50
		20	108.90	105.75	98.45	107.72	5.72
	5-CHQG	5	103.93	83.00	118.07	96.08	14.12
		10	80.15	93.90	107.60	85.31	14.61
		20	81.01	79.83	99.94	80.57	14.64
	5,7-DCHQG	5	96.94	85.53	108.00	92.66	8.70
		10	76.73	100.80	100.33	85.75	18.64
		20	75.59	87.95	98.98	80.23	13.97

^aNIFDS; ^bSRFDA, ^cSGS

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