Table 1 The results of precision (intra, inter-day) and accuracy (recovery) of isolated iridoids1 − 6
Iridoids | Content (mg/g) | Sample | Intra-day (n = 6) | Inter-day (n = 18) | Accuracy (n = 6) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
mg/mL | SD | RSD (%) | SD | RSD (%) | Spiked (mg/g) | Theoretical amount (ug/mL) | Recorded amount (ug/mL) | Recovery (%) | RSD (%) | ||
1 | 85.446 | 1.0 | 8.20 | 1.98 | 1.52 | 1.63 | 50.0 | 135.45 | 138.02 | 101.97 | 0.63 |
2.0 | 16.64 | 1.95 | – | – | 100.0 | 185.45 | 187.12 | 100.95 | 0.89 | ||
4.0 | 9.72 | 1.18 | – | – | 200.0 | 285.45 | 288.25 | 100.98 | 0.50 | ||
2 | 9.361 | 1.0 | 1.59 | 1.75 | 0.19 | 1.93 | 12.5 | 21.86 | 22.04 | 100.82 | 1.03 |
2.0 | 3.66 | 2.00 | – | – | 25.0 | 34.36 | 34.45 | 100.27 | 1.24 | ||
4.0 | 6.05 | 1.64 | – | – | 50.0 | 59.36 | 59.16 | 99.67 | 1.11 | ||
3 | 11.664 | 1.0 | 2.16 | 1.91 | 0.26 | 1.98 | 12.5 | 24.16 | 24.27 | 100.45 | 0.77 |
2.0 | 2.63 | 1.11 | – | – | 25.0 | 36.66 | 36.79 | 100.33 | 1.16 | ||
4.0 | 9.12 | 1.93 | – | – | 50.0 | 61.66 | 60.90 | 98.77 | 1.77 | ||
4 | 9.149 | 1.0 | 1.60 | 1.90 | 0.19 | 1.85 | 12.5 | 21.65 | 21.69 | 100.20 | 0.68 |
2.0 | 3.17 | 1.80 | – | – | 25.0 | 34.15 | 34.18 | 100.08 | 0.80 | ||
4.0 | 3.69 | 1.05 | – | – | 50.0 | 59.15 | 56.18 | 94.97 | 1.59 | ||
5 | 24.186 | 1.0 | 4.61 | 1.96 | 0.21 | 0.87 | 25.0 | 49.19 | 49.14 | 99.93 | 0.80 |
2.0 | 8.60 | 1.77 | – | – | 50.0 | 74.19 | 73.37 | 98.91 | 0.61 | ||
4.0 | 15.72 | 1.68 | – | – | 100.0 | 124.19 | 126.10 | 101.54 | 1.18 | ||
6 | 12.934 | 1.0 | 2.43 | 1.90 | 0.17 | 1.31 | 12.5 | 25.43 | 25.05 | 98.58 | 1.16 |
2.0 | 5.64 | 1.97 | – | – | 25.0 | 37.93 | 37.31 | 98.42 | 0.95 | ||
4.0 | 10.51 | 1.92 | – | – | 50.0 | 66.72 | 67.16 | 100.65 | 0.87 | ||