Assessment of iridoid profiles in the growth period of aerial parts of Pseudolysimachion rotundum var. subintegrum and their antioxidant and MUC5AC inhibitory potential

Applied Biological Chemistry

Table 3 Total phenolic, flavonoid contents, DPPH, ABTS, and MUC5AC inhibition in extracts of P. rotundum var subintegrum at growth stages

P. rotundum	Total phenolic content (mg GAE/100 g)^a	Total flavonoid content (mg CE/100 g)^b	DPPH radical Scavenging activity IC₅₀ (mg/mL)^c	ABTS radical Scavenging activity IC₅₀ (mg/mL)^c	MUC5AC Inhibition rate at 40 µg/mL (%)^d
15 Jul 2021	1178.1 ± 6.2	870.4 ± 1.7	0.49 ± 0.05	1.20 ± 0.04	48.6 ± 0.9
29 Jul 2021	1129.7 ± 37.5	1171.1 ± 22.7	0.52 ± 0.03	1.35 ± 0.09	21.4 ± 1.0
Average	1153.9	1020.8	0.51	1.28	35.0
12 Aug 2021	1304.1 ± 81.7	1227.6 ± 0.7	0.54 ± 0.05	1.38 ± 0.01	17.7 ± 3.5
26 Aug 2021	1442.5 ± 84.4	1313.2 ± 45.8	0.55 ± 0.05	1.49 ± 0.01	50.0 ± 4.7
Average	1373.3	1270.4	0.55	1.44	33.9

^aGallic acid equivalent (GAE) was used as a standard for measuring the total phenolic content
^bCatechin equivalent (CE) was used as a standard for measuring the total flavonoid content
^cEffective activity was expressed as the mean of 50% IC (half maximal inhibitory concentration) of triplicate experiments
^dData presented are the inhibition rate mean ± S.D. of triplicate experiments