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Applied Biological Chemistry
Fig. 4 | Applied Biological Chemistry

Fig. 4

From: Beneficial effects of WON-21 on the symptoms of a hangover and identification of active compounds: experimental studies on antioxidant, anti-inflammation, and alcohol-metabolizing enzymes

Fig. 4

Effect of WON-21 on the expression of iNOS, COX-2, NFκB, and TNF-α levels in RAW 264.7. Representative western blot results and bar graphs show the expression and densitometry ratios of iNOS and COX-2 to GAPDH, and p- NFκB/ NFκB, respectively (A, B). ELISA measurement of TNF‐α levels (C). Data presented the average of three independent ex-periments and was analyzed using one-way ANOVA with Tukey’s post hoc. #p < 0.05 compared with control; *p < 0.05, **p < 0.01 ***p < 0.001 compared with LPS-stimulated cells. iNOS inducible nitric oxide synthase, COX-2 cyclooxygenase-2, NFκB nuclear factor kappa-light-chain-enhancer of activated B cells, TNF‐α tumor necrosis factor‐α

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