Table 2 Analytical conditions for simultaneous determination of 12 marker components in WON-21 sample by HPLC
Operation condition | |||
|---|---|---|---|
Column | SunFire C18 analytical column (250 mm × 4.6 mm, 5 μm) |  |  |
Detector | PDA (250, 280, 290, 325, 335, 350, and 370Â nm) | Â | Â |
Flow rate | 1.0Â mL/min | Â | Â |
Injection volume | 10.0 μL |  |  |
Column temperature | 40.0 °C |  |  |
Mobile phase | A: 0.1% (v/v) aqueous formic acid B: 0.1% (v/v) formic acid in acetonitrile | Â | Â |
| Â | Time (min) | A (%) | B (%) |
|---|---|---|---|
Gradient elution | 0 | 95 | 5 |
60 | 40 | 60 | |
70 | 40 | 60 | |
80 | 95 | 5 | |
90 | 95 | 5 |