Skip to main content
Applied Biological Chemistry
Fig. 4 | Applied Biological Chemistry

Fig. 4

From: Production of omega-5 gliadin monoclonal antibodies for allergenic evaluation of WDEIA-causing wheat varieties

Fig. 4

Analysis of gliadins separated by SDS-PAGE and subsequently stained with Coomassie (a) and immunoblotted with mono-O5B-1C10 (b). The nucleotide sequence of omega-B6 (GenBank accession number AB181300) and its codon sequences, with forward and reverse primers highlighted in yellow and the codon sequences marked in red, representing the target site of the mono-O5B-1C10 antibody (c). Confirmation of omega-B6 gene deletion by PCR analysis (d)

Back to article page