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Table 1 The results of enzyme-linked immunosorbent assay (ELISA) test for selecting first fusion of omega-5 gliadins

From: Production of omega-5 gliadin monoclonal antibodies for allergenic evaluation of WDEIA-causing wheat varieties

Clone No.

O.Da450 nm

Clone No.

O.D450 nm

Omega-5 gliadin (Agb)

SMCCc control

Omega-5 gliadin (Ag)

SMCC control

1C10d

2.572

0.064

6G4

0.317

0.047

1D1

0.287

0.055

6H4

2.686

0.046

2B5

1.43

0.049

7F3

2.304

0.049

3E10

0.145

0.053

8B3

0.047

0.05

3F12

0.046

0.05

8D1

2.36

0.046

3G8

0.157

0.05

8D8

2.468

0.048

3H3

0.406

0.052

8H7

0.25

0.056

3H5

0.096

0.13

8H11

2.565

0.057

4B10

1.245

0.052

9A7

0.491

0.045

4C6

2.192

0.048

9A9

0.477

0.044

4C9

1.372

0.049

9D8

0.155

0.044

4H6

0.543

0.05

9E5

0.048

0.047

4H11

0.045

0.22

9F9

0.803

0.053

5H1

0.19

0.046

10B5

2.282

0.046

6C9

2.401

0.047

10D6

2.31

0.055

6E11

2.475

0.058

10F8

0.269

0.055

6F2

1.133

0.053

Positive

2.677

0.454

   

Negative

0.045

0.045

  1. aOptical density
  2. bAntigen
  3. cSulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)
  4. dBold means 17 clones that specifically reacted with omega-5 gliadins